pine seedling with soil knife. photo credit justine karst

Rehabilitation of Beetle-killed Stands by Improving their Associations with Mycorrhizal Fungi

How do below-ground communities change after MPB attack, and what impact does this have on regeneration?

Previous research demonstrates that fungal communities in soils change following beetle-induced stand mortality with consequences for pine seedling survival and performance. This next stage of research will investigate how community profiles of fungi in soils of beetle-killed stands vary by ecosite, and whether inoculation of pine seedlings with fungi from un-attacked pine stands can offset the effects of detrimental fungi occurring in soils of some beetle-killed stands.

This research will allow understanding of below-ground factors and their impact on seedling growth, defense and survival following stand death by MPB. The objectives are to:

  1. Use molecular techniques to survey community profiles of soil fungi occurring in beetle-killed stands over a range of ecosites
  2. use field experiments to assess whether pine seedling performance is altered by inoculation by soil fungi from different origins (beetle-killed versus undisturbed mature pine stands), and
  3. compare fungal community profiles between sites disturbed by fire and mountain pine beetle.
January 2016
Project Begins

Planning, led by Dr. Justine Karst, is underway.

May 2016
Project Presented

Dr. Karst presents the project plan at the Information Forum

Summer 2016
Surveys Begin

Soil in stands with high pine mortality collected for DNA analysis of fungal communities

Winter 2016–2017
Communities Characterized

Biotic and abiotic factors in soil are assessed and compared between disturbance types and ecosites

Winter 2017
Seedlings Innoculated

Lodgepole pine seedlings inoculated with fungus in a greenhouse

Summer 2017
Seedlings Outplanted

Based on sites identified in the winter, inoculated seedlings are planted in a range of soil environments

June 2017
Objective 1 Results Published

Peer-reviewed paper in New Phytologist

Summer 2018
Seedlings Measured

Outplanted seedlings are assessed for growth and survival

2019
Analysis and Communication Underway

Reports and QuickNotes delivered

February 2020
Paper Published

Peer-reivewed paper in Mycorrhiza

March 2020
Project Complete
Videos | Audio-Visual
Presentation from the 2016 fRI Research Mountain Pine Beetle Ecology Program's Research Forum & Field Tour
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Dr. Justine Karst
Dr. Justine Karst
Dr. Nadir Erbilgin
Board Member
Dr. Keith McClain
Dr. Keith McClain
Program Lead